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scramble control sgrna  (Addgene inc)


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    Structured Review

    Addgene inc scramble control sgrna
    Scramble Control Sgrna, supplied by Addgene inc, used in various techniques. Bioz Stars score: 96/100, based on 1086 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/scramble+control+sgrna/scramble+shRNA+(Plasmid+%231864)/pmc11228649__mmc2-631-0-18
    Average 96 stars, based on 1086 article reviews
    scramble control sgrna - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Control:

    Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML
    Article Snippet: In the sorting experiment, the stained cells were sorted out with a cell sorter (BD FACSAria). shRNA sequences, sgRNA sequences, and DNA constructs Scramble control shRNA or targeting shRNA, designed by an online tool (https://portals.broadinstitute.org/gpp/public/seq/search), were cloned into pLKO.5 plasmid (Sigma-Aldrich, SHC201), individually. .. Scramble control sgRNA or targeting sgRNA, designed by an online tool (https://portals.broadinstitute.org/gppx/crispick/public), were cloned into LentiGuide Puro-P2A-EGFP plasmid (addgene, #137729; a gift from Fredrik Wermeling), individually. .. Cd36 cDNA, Cd36 mutant cDNA, OVA cDNA, IkB-SR cDNA, MYD88-GyrB cDNA, MYD88 mutant-GyrB cDNA, were cloned into pLV-EF1a-IRES-Neo plasmid (addgene, #85139; a gift from Tobias Meyer), individually. shRNA sequences and sgRNA sequences are listed in Table S4.

    Clone Assay:

    Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML
    Article Snippet: In the sorting experiment, the stained cells were sorted out with a cell sorter (BD FACSAria). shRNA sequences, sgRNA sequences, and DNA constructs Scramble control shRNA or targeting shRNA, designed by an online tool (https://portals.broadinstitute.org/gpp/public/seq/search), were cloned into pLKO.5 plasmid (Sigma-Aldrich, SHC201), individually. .. Scramble control sgRNA or targeting sgRNA, designed by an online tool (https://portals.broadinstitute.org/gppx/crispick/public), were cloned into LentiGuide Puro-P2A-EGFP plasmid (addgene, #137729; a gift from Fredrik Wermeling), individually. .. Cd36 cDNA, Cd36 mutant cDNA, OVA cDNA, IkB-SR cDNA, MYD88-GyrB cDNA, MYD88 mutant-GyrB cDNA, were cloned into pLV-EF1a-IRES-Neo plasmid (addgene, #85139; a gift from Tobias Meyer), individually. shRNA sequences and sgRNA sequences are listed in Table S4.

    Plasmid Preparation:

    Article Title: A CD36-dependent non-canonical lipid metabolism program promotes immune escape and resistance to hypomethylating agent therapy in AML
    Article Snippet: In the sorting experiment, the stained cells were sorted out with a cell sorter (BD FACSAria). shRNA sequences, sgRNA sequences, and DNA constructs Scramble control shRNA or targeting shRNA, designed by an online tool (https://portals.broadinstitute.org/gpp/public/seq/search), were cloned into pLKO.5 plasmid (Sigma-Aldrich, SHC201), individually. .. Scramble control sgRNA or targeting sgRNA, designed by an online tool (https://portals.broadinstitute.org/gppx/crispick/public), were cloned into LentiGuide Puro-P2A-EGFP plasmid (addgene, #137729; a gift from Fredrik Wermeling), individually. .. Cd36 cDNA, Cd36 mutant cDNA, OVA cDNA, IkB-SR cDNA, MYD88-GyrB cDNA, MYD88 mutant-GyrB cDNA, were cloned into pLV-EF1a-IRES-Neo plasmid (addgene, #85139; a gift from Tobias Meyer), individually. shRNA sequences and sgRNA sequences are listed in Table S4.



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    CTD serine 2, but not serine 7, is required for efficient viral gene expression. HEK-293T cells were co-transfected with a construct expressing a <t>sgRNA</t> transcribed by <t>Pol</t> <t>III</t> as a transfection control and plasmids expressing amanitin-resistant RPB1 with wild-type (WT) or indicated mutant CTDs, or the control vector pcDNA3 lacking any polymerase. The next day, α-amanitin was added to degrade endogenous RPB1 for 24 h followed by infection with HSV-1 strain 17+, and viral gene expression was measured by RT-qPCR at 24 hpi. Means of three biological replicates with standard error are plotted. Statistically significant differences to mock are indicated as * P < 0.05, ** P < 0.01, *** P < 0.001. S2A: serine 2 to alanine, S7A: serine 7 to alanine, S7E: serine 7 to glutamate.
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    Addgene inc control scrambled sgrna crispr lenti vector
    CTD serine 2, but not serine 7, is required for efficient viral gene expression. HEK-293T cells were co-transfected with a construct expressing a <t>sgRNA</t> transcribed by <t>Pol</t> <t>III</t> as a transfection control and plasmids expressing amanitin-resistant RPB1 with wild-type (WT) or indicated mutant CTDs, or the control vector pcDNA3 lacking any polymerase. The next day, α-amanitin was added to degrade endogenous RPB1 for 24 h followed by infection with HSV-1 strain 17+, and viral gene expression was measured by RT-qPCR at 24 hpi. Means of three biological replicates with standard error are plotted. Statistically significant differences to mock are indicated as * P < 0.05, ** P < 0.01, *** P < 0.001. S2A: serine 2 to alanine, S7A: serine 7 to alanine, S7E: serine 7 to glutamate.
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    Image Search Results


    CTD serine 2, but not serine 7, is required for efficient viral gene expression. HEK-293T cells were co-transfected with a construct expressing a sgRNA transcribed by Pol III as a transfection control and plasmids expressing amanitin-resistant RPB1 with wild-type (WT) or indicated mutant CTDs, or the control vector pcDNA3 lacking any polymerase. The next day, α-amanitin was added to degrade endogenous RPB1 for 24 h followed by infection with HSV-1 strain 17+, and viral gene expression was measured by RT-qPCR at 24 hpi. Means of three biological replicates with standard error are plotted. Statistically significant differences to mock are indicated as * P < 0.05, ** P < 0.01, *** P < 0.001. S2A: serine 2 to alanine, S7A: serine 7 to alanine, S7E: serine 7 to glutamate.

    Journal: Journal of Virology

    Article Title: Herpes simplex virus 1 inhibits phosphorylation of RNA polymerase II CTD serine-7

    doi: 10.1128/jvi.01178-24

    Figure Lengend Snippet: CTD serine 2, but not serine 7, is required for efficient viral gene expression. HEK-293T cells were co-transfected with a construct expressing a sgRNA transcribed by Pol III as a transfection control and plasmids expressing amanitin-resistant RPB1 with wild-type (WT) or indicated mutant CTDs, or the control vector pcDNA3 lacking any polymerase. The next day, α-amanitin was added to degrade endogenous RPB1 for 24 h followed by infection with HSV-1 strain 17+, and viral gene expression was measured by RT-qPCR at 24 hpi. Means of three biological replicates with standard error are plotted. Statistically significant differences to mock are indicated as * P < 0.05, ** P < 0.01, *** P < 0.001. S2A: serine 2 to alanine, S7A: serine 7 to alanine, S7E: serine 7 to glutamate.

    Article Snippet: For qPCR analysis, the assay was repeated but scaled up to 24-well plates, this time using 50 ng of the vector pU6.scr_sgRNA in place of Azurite, which expresses a scrambled control sgRNA based on Addgene #62285 from the RNA Pol III promoter U6.

    Techniques: Gene Expression, Transfection, Construct, Expressing, Control, Mutagenesis, Plasmid Preparation, Infection, Quantitative RT-PCR